ABSTRACT
Gastric Cancer (GC) is one of the most dangerous malignancies in the world. This study aims to evaluate the relationship between miR-146a and miR-155 in patients with H. pylori infections with GC compared to H. pylori-infected patients and healthy subjects. Forty patients with H. pylori and GC positive diagnoses and 40 patients with H. pylori positive and GC negative diagnoses, and 40 healthy persons were selected.The expression of miR-146a and miR-155 genes in the whole blood was examined using qRT-PCR. Moreover, ROC curves were drawn to represent the sensitivity and specificity of miR-146a and miR-155 expression as biomarkers. The results showed the expression of miR-146a and miR-155 in the whole blood of patients with H. pylori and GC positive diagnoses are significantly higher than in healthy individuals and are non-significantly enhanced compared to H. pylori positive and GC negative. Also, the results stated miR-146a and miR-155 expression in the whole blood of patients who are H. pylori positive and GC negative are significantly increased compared to healthy individuals. Furthermore, the ROC curve analysis of miR-146a and miR-155 RNA level demonstrated the two miRNAs have an appropriate sensitivity and specificity for diagnostic goals. In conclusion, H. pylori infection may increase the expression of miR-146a and miR-155 in patients with H. pylori and GC positive diagnoses, which can be effective in the curbing the progression of GC. For this reason, up-regulation of miR-146a and miR-155 along with H. pylori infection might contribute to the pathogenesis of GC, and also can be suggested as biomarkers for GC diagnosis and treatment.
ABSTRACT
@#Introduction: Sleep disorder is one of the most common problems in elderly people. The present study is focused on the effectiveness of educational intervention on sleep quality and psychological well-being in the elderly people. Method: This is a Randomized educational field-controlled trial which was conducted on 90 elderlies with the sleep disorder, who have been selected using systematic random sampling from the clients of comprehensive health centers in Borazjan, Iran, 2019. The educational intervention was presented for 5 weeks by lecture, group discussions and virtual learning methods. Data were collected by the Pittsburgh Sleep Quality Index and the Ryff’s Psychological well-being scale before and 2 months after the intervention. The data were analyzed using SPSS-25 software using independent t-test, and Chi-square test. Results: The results showed significant statistical differences in sleep quality, subscales of sleep efficiency, sleeping time, and taking sleeping pills in experimental and control groups after the intervention (p≤0.005). Moreover, significant statistical difference was observed in terms of the mean scale of psychological well-being between experimental and control groups (p=0.036). Conclusion: Behavioral interventions can make various advantages for elderly people. Therefore, this study suggests providing such interventions in a wide range in comprehensive health centers, and boarding centers for the elderly.